We’re using up so much OADC (Oleic acid, Albumin, Dextrose, Catalase) to grow our mycobacteria on agar that we decided to prepare the expensive OADC supplement ourselves!
For 1L: 50g Albumin Fraction V, 0.6mL oleic acid ( we used #75090-5mL from SIGMA) resuspended first in 50mL diH2O containing 0.05N NaOH, 20g dextrose, 8.5g NaCl and 30mg catalase (we used #C1345-1G from SIGMA) – diH2O up to 1L – Mixing by magnetic stirring and low heating (<50°C) – Adjust pH to 6.9-7.0 – Filter sterilize 0.22µm, aliquot by 50 or 100mL and store at 4°C for about 6 months max.